payload: {"action":"closed","issue":{"url":"https://api.github.com/repos/biosafetylvl5/tdt-characterization/issues/9","repository_url":"https://api.github.com/repos/biosafetylvl5/tdt-characterization","labels_url":"https://api.github.com/repos/biosafetylvl5/tdt-characterization/issues/9/labels{/name}","comments_url":"https://api.github.com/repos/biosafetylvl5/tdt-characterization/issues/9/comments","events_url":"https://api.github.com/repos/biosafetylvl5/tdt-characterization/issues/9/events","html_url":"https://github.com/biosafetylvl5/tdt-characterization/issues/9","id":411260199,"node_id":"MDU6SXNzdWU0MTEyNjAxOTk=","number":9,"title":"check TdT stocks in UTL","user":{"login":"hyacynth","id":47726948,"node_id":"MDQ6VXNlcjQ3NzI2OTQ4","avatar_url":"https://avatars0.githubusercontent.com/u/47726948?v=4","gravatar_id":"","url":"https://api.github.com/users/hyacynth","html_url":"https://github.com/hyacynth","followers_url":"https://api.github.com/users/hyacynth/followers","following_url":"https://api.github.com/users/hyacynth/following{/other_user}","gists_url":"https://api.github.com/users/hyacynth/gists{/gist_id}","starred_url":"https://api.github.com/users/hyacynth/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/hyacynth/subscriptions","organizations_url":"https://api.github.com/users/hyacynth/orgs","repos_url":"https://api.github.com/users/hyacynth/repos","events_url":"https://api.github.com/users/hyacynth/events{/privacy}","received_events_url":"https://api.github.com/users/hyacynth/received_events","type":"User","site_admin":false},"labels":[{"id":1238699214,"node_id":"MDU6TGFiZWwxMjM4Njk5MjE0","url":"https://api.github.com/repos/biosafetylvl5/tdt-characterization/labels/Real%20Life%20Problem","name":"Real Life Problem","color":"6094bc","default":false}],"state":"closed","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":1,"created_at":"2019-02-18T00:57:55Z","updated_at":"2019-02-18T23:56:00Z","closed_at":"2019-02-18T23:56:00Z","author_association":"NONE","body":"your current design uses 2uL*88 wells of TdT = 176 uL at least, probably more with pipetting error. largest size tube of TdT from NEB (product number M0315L) contains 125 uL of TdT, and all of ours are probably partially used. You're going to need to order at least 2 fresh tubes of TdT (more if you want to scale your reaction volumes up) or check shriram to see how much tdt we have left in stock if you want to risk mixing multiple tubes of old tdt together (would be kinda sketch)."}}
other: {"actor":{"display_login":"biosafetylvl5"}}