payload: {"action":"created","issue":{"url":"https://api.github.com/repos/DamienFr/GC_content_in_sliding_window/issues/6","repository_url":"https://api.github.com/repos/DamienFr/GC_content_in_sliding_window","labels_url":"https://api.github.com/repos/DamienFr/GC_content_in_sliding_window/issues/6/labels{/name}","comments_url":"https://api.github.com/repos/DamienFr/GC_content_in_sliding_window/issues/6/comments","events_url":"https://api.github.com/repos/DamienFr/GC_content_in_sliding_window/issues/6/events","html_url":"https://github.com/DamienFr/GC_content_in_sliding_window/issues/6","id":547220347,"node_id":"MDU6SXNzdWU1NDcyMjAzNDc=","number":6,"title":"can plant genome suitable","user":{"login":"wuxiaopei0509","id":52643750,"node_id":"MDQ6VXNlcjUyNjQzNzUw","avatar_url":"https://avatars0.githubusercontent.com/u/52643750?v=4","gravatar_id":"","url":"https://api.github.com/users/wuxiaopei0509","html_url":"https://github.com/wuxiaopei0509","followers_url":"https://api.github.com/users/wuxiaopei0509/followers","following_url":"https://api.github.com/users/wuxiaopei0509/following{/other_user}","gists_url":"https://api.github.com/users/wuxiaopei0509/gists{/gist_id}","starred_url":"https://api.github.com/users/wuxiaopei0509/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/wuxiaopei0509/subscriptions","organizations_url":"https://api.github.com/users/wuxiaopei0509/orgs","repos_url":"https://api.github.com/users/wuxiaopei0509/repos","events_url":"https://api.github.com/users/wuxiaopei0509/events{/privacy}","received_events_url":"https://api.github.com/users/wuxiaopei0509/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":1,"created_at":"2020-01-09T03:06:09Z","updated_at":"2020-01-09T07:08:30Z","closed_at":null,"author_association":"NONE","body":"My Genome is a 700Mb plant genome, can I use this script to obtain GC content?"},"comment":{"url":"https://api.github.com/repos/DamienFr/GC_content_in_sliding_window/issues/comments/572422754","html_url":"https://github.com/DamienFr/GC_content_in_sliding_window/issues/6#issuecomment-572422754","issue_url":"https://api.github.com/repos/DamienFr/GC_content_in_sliding_window/issues/6","id":572422754,"node_id":"MDEyOklzc3VlQ29tbWVudDU3MjQyMjc1NA==","user":{"login":"DamienFr","id":13535733,"node_id":"MDQ6VXNlcjEzNTM1NzMz","avatar_url":"https://avatars3.githubusercontent.com/u/13535733?v=4","gravatar_id":"","url":"https://api.github.com/users/DamienFr","html_url":"https://github.com/DamienFr","followers_url":"https://api.github.com/users/DamienFr/followers","following_url":"https://api.github.com/users/DamienFr/following{/other_user}","gists_url":"https://api.github.com/users/DamienFr/gists{/gist_id}","starred_url":"https://api.github.com/users/DamienFr/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/DamienFr/subscriptions","organizations_url":"https://api.github.com/users/DamienFr/orgs","repos_url":"https://api.github.com/users/DamienFr/repos","events_url":"https://api.github.com/users/DamienFr/events{/privacy}","received_events_url":"https://api.github.com/users/DamienFr/received_events","type":"User","site_admin":false},"created_at":"2020-01-09T07:08:30Z","updated_at":"2020-01-09T07:08:30Z","author_association":"OWNER","body":"I do not see why it wouldn't work.\r\n\r\nIt doesn't impact much the result, but it worth mentioning that this tool is made for circular genomes, so the values of GC_content of the beginning of your sequence are calculated taking into account nucleotides at the end of the sequence as well.\r\n\r\ne. g.\r\nWith a window size of 1000, the calculation of the GC content of the first base of the sequence depends on the first 500 bp and the last 500bp of your sequence.\r\n\r\n"}}
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